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1.
Rev. bras. med. esporte ; 23(1): 55-59, jan.-fev. 2017. tab, graf
Article in English | LILACS | ID: biblio-843967

ABSTRACT

ABSTRACT Introduction: Peripheral nerve adaptation is critical for strength gains. However, information about intensity effects on nerve morphology is scarce. Objective: To compare the effects of different intensities of resistance training on radial nerve structures. Methods: Rats were divided into three groups: control (GC), training with 50% (GF1) and training 75% (GF2) of the animal’s body weight. The morphological analysis of the nerve was done by light and transmission electron microscopy. One-way ANOVA and the Tukey’s post hoc test were applied and the significance level was set at p≤0.05. Results: Training groups had an increase of strength compared to GC (p≤0.05). All measured nerve components (mean area and diameter of myelin fibers and axons, mean area and thickness of the myelin sheath, and of neurofilaments and microtubules) were higher in GF2 compared to the other (p≤0.05). Conclusion: Results demonstrated greater morphological changes on radial nerve after heavier loads. This can be important for rehabilitation therapies, training, and progression.


RESUMO Introdução: A adaptação dos nervos periféricos é fundamental para ganhos de força. No entanto, as informações relativas aos efeitos da intensidade sobre a morfologia do nervo são escassas. Objetivo: Comparar os efeitos de diferentes intensidades de treinamento de resistência sobre estruturas do nervo radial. Métodos: Os ratos foram divididos em três grupos: controle (GC) e treinamento com 50% (GF1) e 75% (GF2) do peso corporal do animal. A análise morfológica do nervo foi feita com microscopia óptica e eletrônica de transmissão. A one-way ANOVA e o teste post hoc de Tukey foram aplicados e o nível de significância foi estabelecido em p ≤ 0,05. Resultados: Os grupos treinamento tiveram aumento de força com relação ao GC (p ≤ 0,05). Todos os componentes medidos do nervo (área média e diâmetro de fibras de mielina e axônios, área média e espessura da bainha de mielina, neurofilamentos e microtúbulos) foram maiores no GF2 em comparação com os demais (p ≤ 0,05). Conclusão: Os resultados demonstraram maiores alterações morfológicas no nervo radial depois de cargas mais pesadas. Isso pode ser importante para terapias de reabilitação, treinamento e progressão.


RESUMEN Introducción: La adaptación de los nervios periféricos es fundamental para el aumento de fuerza. Sin embargo, la información sobre el efecto de la intensidad sobre la morfología del nervio es escasa. Objetivo: Comparar el efecto de diferentes intensidades de entrenamiento de resistencia en las estructuras del nervio radial. Métodos: Las ratas se dividieron en tres grupos: control (GC) y entrenamiento con 50% (GF1) y con 75% (GF2) del peso corporal del animal. El análisis morfológico del nervio se hizo con microscopía óptica y electrónica de transmisión. Se aplicaron la prueba ANOVA de una vía y la prueba post hoc de Tukey y el nivel de significación se fijó en p ≤ 0,05. Resultados: Los grupos de entrenamiento tuvieron aumento de la fuerza con respecto al grupo control (p ≤ 0,05). Todos los componentes medidos del nervio (área media y diámetro de las fibras de mielina y axones, área media y espesor de la vaina de mielina, neurofilamentos y microtúbulos) fueron mayores en GF2 en comparación con los otros grupos (p ≤ 0,05). Conclusión: Los resultados mostraron mayores cambios morfológicos en el nervio radial después de las cargas más pesadas. Esto puede ser importante para terapias de rehabilitación, entrenamiento y progresión.

2.
Conscientiae saúde (Impr.) ; 14(2): 195-199, 30 jun. 2015.
Article in English | LILACS | ID: biblio-736

ABSTRACT

Objective: To determine the effective remnant power after the passage of low intensity laser of two different wavelengths (660 and 780 nm) through buccinator muscle in anatomical hemifaces. Methods: Five human hemifaces were dissected, and a laser was shone in the central region of the muscle with a receiver placed on the opposite side. The hemifaces were irradiated using the same dosimetric parameters (40 mW, 1 W/cm2, continuous mode, 0.04 cm2) and at the same point in every muscle; remnant power was measured for each irradiation. Results: The average remnant power after irradiation with 660 nm (149 ± 15 µW) light was significantly lower (p<0.0001) than with that of 780 nm (380 ± 40 µW). Conclusion: The LIL of 780 nm presented greater remnant power in comparison to the 660 nm laser after passage through the buccinator muscle, indicating the latter ́s greater penetration capacity.


Objetivo: Determinar a potência remanescente após a passagem do laser de baixa intensidade em dois comprimentos de onda (660 e 780 nm) através do músculo bucinador em peças anatômicas dissecadas. Métodos: Dissecaram-se cinco hemifaces humanas expondo o músculo bucinador. Aplicou-se o laser na região central de cada músculo, e o receptor, para aferir a potência remanescente, foi posicionado na superfície intraoral contrária. As hemifaces foram irradiadas nos mesmos parâmetros dosimétricos (40 mW, 1 W/cm2, modo contínuo, 0,04 cm2) e no mesmo ponto em cada músculo, sendo a potência remanescente aferida a cada repetição. Resultados: A potência média restante após as irradiações de 660 nm (149 ± 15 µW) foi menor (p <0,0001) que com as irradiações de 780 nm (380 ± 40 µW). Conclusão: O LBI de 780 nm apresentou maior potência remanescente que o LBI de 660 nm, após passagem pelo músculo bucinador, denotando sua maior capacidade de penetração.


Subject(s)
Humans , Low-Level Light Therapy , Facial Muscles , Myofascial Pain Syndromes/radiotherapy , Dissection
3.
Braz. oral res ; 27(5): 438-444, Sep-Oct/2013. tab, graf
Article in English | LILACS | ID: lil-685420

ABSTRACT

Primary teeth are interesting models that can be used to study physiological and pathological processes involving cells and extracellular matrices in hard and soft tissues. This study investigated the expression and distribution of biglycan and decorin-the non-collagenous components of the extracellular matrix-in primary teeth tissue, during physiological root resorption. Thirty healthy human primary teeth were grouped together according to root length: Group I - two-thirds root length, Group II - one-third root length, and Group III - teeth with no root. The streptavidin-biotin-peroxidase immunohistochemical method was used with antibodies against the previously named antigens. The proteoglycans studied were found in the pulp and dentin extracellular matrix in all groups without any differences in the proteins, among the groups. Biglycan was observed mainly in predentin and in pulp connective tissue in the resorption area. In addition, decorin was observed mainly in pulp connective tissue, but near the resorption area. Biglycan and decorin were distributed differentially in the dental tissues. The present immunohistocytochemical data, combined with previously reported data, suggest that these proteoglycans could be involved in regulating the physiological resorption process in healthy primary teeth.


Subject(s)
Child , Humans , Biglycan/analysis , Decorin/analysis , Dental Pulp/metabolism , Root Resorption/physiopathology , Tooth, Deciduous/metabolism , Biglycan/metabolism , Decorin/metabolism , Dental Pulp/cytology , Dentin/chemistry , Dentin/metabolism , Extracellular Matrix/metabolism , Immunohistochemistry , Statistics, Nonparametric , Tooth, Deciduous/cytology
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